Article 13420

Title of the article

THE STUDY OF ANTIBACTERIAL COATING EFFECT FOR IMPLANTABLE MEDICAL DEVICES MADE OF TITANIUM AND CHANGES IN HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF RAT BLOOD AT DIFFERENT PERIODS AFTER IMPLANTATION 

Authors

Ivanova Valeriya Eduardovna, Student, Penza State University (40 Krasnaya street, Penza, Russia), E-mail: ivanovavaleria102@gmail.com
Andreeva Elena Sergeevna, Student, Penza State University (40 Krasnaya street, Penza, Russia), E-mail: andreevalena58@gmail.com
Kruchinina Anastasiya Dmitrievna, Candidate of biological sciences, associate professor, sub-department of general biology and biochemistry, Penza State University (40 Krasnaya street, Penza, Russia), E-mail: a.d.kruchinina@mail.ru
Gamzin Sergey Sergeevich, Candidate of biological sciences, associate professor, sub-department of general biology and biochemistry, Penza State University (40 Krasnaya street, Penza, Russia), E-mail: s.s.gamzin@yandex.ru 

Index UDK

616-77 

DOI

10.21685/2072-3032-2020-4-13 

Abstract

Background. Despite the annual increase in the number of operations using titanium alloy products, the problem of developing periprosthetic infectious complications remains unresolved. The promising direction in traumatology and orthopedics is the creation of implants that prevent the formation of biofilm on the surface of the medical device. It was proposed to apply an antibacterial coating to the implant surface, including vancomycin as the active component and the polymer polylactideco-glycolide as the carrier. The aim of the research was to study the antibacterial effect of the coating titanium blocks in vitro and analyze the dynamics of changes in the level of basic hematological and biochemical parameters at different periods after implantation in rats.
Materials and methods. The disc-diffusion test was used to evaluate the antibacterial effect of the coating on Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis cultures. Samples with the antibacterial coating were implanted subcutaneously, and changes in the hematological and biochemical parameters of rat blood were studied in dynamics. Statistical data processing was performed using Tukey’s test, the Shapiro–Wilk test, the Kruskal-Wallis test, and the Mann-Whitney U-test.
Results. In vitro experiments confirmed the presence of an antibacterial effect of the titanium blocks vancomycin-containing coating on cultures of Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis. The results in vivo experiments indicate that the hematological and biochemical parameters of rat blood before surgery, 2 and 4 weeks after implantation of titanium blocks are within the physiological norm. There were no statistically significant differences between the values of the control and experimental groups. The established value shifts reflect the body's response to anesthesia and surgery.
Conclusions. Antibacterial coating for implantable medical devices made of titanium, which contain vancomycin, and polylactide-co-glycolide (50:50) has an antibacterial effect against Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis cultures. The results of the study of hematological and biochemical parameters of the control and experimental groups indicate that there is no toxic effect of the antibacterial coating of titanium blocks on the body.

Key words

implantable medical devices, titanium alloys, periprosthetic infection, antibacterial coating 

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Дата создания: 20.01.2021 09:53
Дата обновления: 20.01.2021 11:23